diff --git a/files/gitlab-cookbooks/gitlab/templates/default/puma.rb.erb b/files/gitlab-cookbooks/gitlab/templates/default/puma.rb.erb
index 541f98caa55d2fd3a83f8d3861bd4369d0fd43ed_ZmlsZXMvZ2l0bGFiLWNvb2tib29rcy9naXRsYWIvdGVtcGxhdGVzL2RlZmF1bHQvcHVtYS5yYi5lcmI=..dbde246b6b68d7a7703af37d29995a06b0425e63_ZmlsZXMvZ2l0bGFiLWNvb2tib29rcy9naXRsYWIvdGVtcGxhdGVzL2RlZmF1bHQvcHVtYS5yYi5lcmI= 100644
--- a/files/gitlab-cookbooks/gitlab/templates/default/puma.rb.erb
+++ b/files/gitlab-cookbooks/gitlab/templates/default/puma.rb.erb
@@ -112,13 +112,6 @@
 # https://github.com/puma/puma/blob/master/5.0-Upgrade.md#lower-latency-better-throughput
 wait_for_less_busy_worker ENV.fetch('PUMA_WAIT_FOR_LESS_BUSY_WORKER', 0.001).to_f
 
-# nakayoshi_fork was removed in Puma 6.0: https://github.com/puma/puma/issues/2258
-# https://github.com/puma/puma/blob/master/5.0-Upgrade.md#nakayoshi_fork
-if Gem::Version.new(Puma::Const::PUMA_VERSION).canonical_segments.first == 5 &&
-     ENV['DISABLE_PUMA_NAKAYOSHI_FORK'] != 'true'
-  nakayoshi_fork
-end
-
 # Use customised JSON formatter for Puma log
 require_relative "<%= @install_dir %>/embedded/service/gitlab-rails/lib/gitlab/puma_logging/json_formatter"